Abstract
Yeast is a powerful genetic model system, but its rigid cell wall has prohibited microinjection. Using microfabricated channels to constrain the fission yeast Schizosaccharomyces pombe, we sheared local regions of individual cells with a piezoelectric unit. The cells remained viable, we detected actin patches in the cell after introduction of fluorescent phalloidin into the medium, and the cytokinetic ring was disrupted after injection of the myosin II inhibitor blebbistatin.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Fluorescent Dyes / administration & dosage
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Heterocyclic Compounds, 4 or More Rings / administration & dosage
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Microinjections / instrumentation
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Microinjections / methods*
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Microscopy, Fluorescence
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Microscopy, Phase-Contrast
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Phalloidine / administration & dosage
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Schizosaccharomyces / drug effects
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces / ultrastructure*
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Schizosaccharomyces pombe Proteins / metabolism
Substances
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Actins
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Fluorescent Dyes
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Heterocyclic Compounds, 4 or More Rings
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Schizosaccharomyces pombe Proteins
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Phalloidine
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blebbistatin