Homo-D-lactic acid fermentation from arabinose by redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-lactate dehydrogenase gene-deficient Lactobacillus plantarum

Kenji Okano; Shogo Yoshida; Tsutomu Tanaka; Chiaki Ogino; Hideki Fukuda; Akihiko Kondo

doi:10.1128/AEM.00573-09

Homo-D-lactic acid fermentation from arabinose by redirection of the phosphoketolase pathway to the pentose phosphate pathway in L-lactate dehydrogenase gene-deficient Lactobacillus plantarum

Appl Environ Microbiol. 2009 Aug;75(15):5175-8. doi: 10.1128/AEM.00573-09. Epub 2009 Jun 5.

Authors

Kenji Okano¹, Shogo Yoshida, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda, Akihiko Kondo

Affiliation

¹ Department of Chemical Science and Engineering, Kobe University, Rokkodaicho, Nada-ku, Japan.

Abstract

Optically pure d-lactic acid fermentation from arabinose was achieved by using the Lactobacillus plantarum NCIMB 8826 strain whose l-lactate dehydrogenase gene was deficient and whose phosphoketolase gene was substituted with a heterologous transketolase gene. After 27 h of fermentation, 38.6 g/liter of d-lactic acid was produced from 50 g/liter of arabinose.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehyde-Lyases / metabolism
Arabinose / metabolism*
Fermentation
Genetic Engineering / methods*
Humans
L-Lactate Dehydrogenase / deficiency
Lactic Acid / metabolism*
Lactobacillus plantarum / genetics*
Lactobacillus plantarum / metabolism*
Metabolic Networks and Pathways / genetics*
Models, Biological
Pentose Phosphate Pathway / genetics

Substances

Lactic Acid
Arabinose
L-Lactate Dehydrogenase
Aldehyde-Lyases
phosphoketolase