Elicitation studies in cell suspension cultures of Cannabis sativa L

J Biotechnol. 2009 Aug 20;143(2):157-68. doi: 10.1016/j.jbiotec.2009.05.006. Epub 2009 Jun 11.

Abstract

Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with biotic and abiotic elicitors to evaluate their effect on secondary metabolism. Metabolic profiles analysed by (1)H NMR spectroscopy and principal component analysis (PCA) showed variations in some of the metabolite pools. However, no cannabinoids were found in either control or elicited cannabis cell cultures. Tetrahydrocannabinolic acid (THCA) synthase gene expression was monitored during a time course. Results suggest that other components in the signaling pathway can be controlling the cannabinoid pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Cannabis / drug effects
  • Cannabis / enzymology*
  • Cannabis / metabolism
  • Cell Culture Techniques
  • Cells, Cultured
  • Cyclopentanes / pharmacology
  • Dronabinol / analogs & derivatives*
  • Dronabinol / metabolism
  • Intramolecular Oxidoreductases / genetics
  • Intramolecular Oxidoreductases / metabolism
  • Metabolome / drug effects
  • Metabolomics / methods
  • Methanol / chemistry
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Oxylipins / pharmacology
  • Pectins / pharmacology
  • Plant Proteins / metabolism*
  • Principal Component Analysis / methods
  • Water / chemistry

Substances

  • Cyclopentanes
  • Oxylipins
  • Plant Proteins
  • Water
  • 11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid
  • jasmonic acid
  • Dronabinol
  • Pectins
  • Intramolecular Oxidoreductases
  • delta(1)-tetrahydrocannabinolic acid synthase
  • Methanol