Development of a multiplex real-time PCR for detection and differentiation of malignant catarrhal fever viruses in clinical samples

J Clin Microbiol. 2009 Aug;47(8):2586-9. doi: 10.1128/JCM.00997-09. Epub 2009 Jun 3.

Abstract

A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • Herpesviridae / classification
  • Herpesviridae / genetics
  • Herpesviridae / isolation & purification*
  • Herpesviridae Infections / diagnosis
  • Herpesviridae Infections / veterinary*
  • Humans
  • Lymphoproliferative Disorders / diagnosis
  • Lymphoproliferative Disorders / veterinary*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Ruminants / virology*
  • Sensitivity and Specificity
  • Sequence Alignment

Substances

  • DNA Primers