Abstract
HBc-VLP can be used in an epitope presentation system to carry foreign epitopes and mimic live virus in order to study viral particle uptake, virion-mediated activation and antigen presentation by dendritic cells. In this study, a multifunctional mAb was produced using a novel research strategy. A truncated HBc-VLP bone vector with a special conformation was used as an immunogen and the target hybridoma cell lines were screened by a series of tests; including ELISA, Western blot, and cellular immunofluorescence based on the epitope presentation system. The screened monoclonal antibody was used to identify the HBc-VLP vector, a fusion HBc-VLP vaccine, and intracellular HBV capsids. The new strategy facilitated acquisition of the desired mAbs and will serve as a reference for other VLP-related research.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / blood
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Antibodies, Monoclonal / immunology*
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Antibodies, Viral / blood
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Antibodies, Viral / immunology*
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Antigen Presentation*
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Cell Line
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Cell Line, Tumor
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Epitopes / chemistry
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Epitopes / genetics
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Epitopes / immunology*
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Female
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Hepatitis B Core Antigens / genetics
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Hepatitis B Core Antigens / immunology*
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Hepatitis B Core Antigens / metabolism*
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Hepatitis B virus / genetics
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Hepatitis B virus / immunology*
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Humans
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Immunization
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Macrophages
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Mice
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Mice, Inbred BALB C
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Protein Conformation
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
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Viral Vaccines / immunology
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Virion / immunology*
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Epitopes
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Hepatitis B Core Antigens
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Recombinant Proteins
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Viral Vaccines