Abstract
Human C21orf63 is a type-1 transmembrane protein of hitherto unknown function, with two repeats of putative 'galactose-binding lectin domains'. By using glycan microarray analysis and other assays, we found that human C21orf63 interacts with heparin and to a lesser extent with heparan sulphate. The C-terminal galactose-binding lectin domain of C21orf63 is necessary for heparin binding. The inability of other human proteins with galactose-binding lectin domains to interact with heparin suggests that heparin binding is a unique property of C21orf63. Results of real-time polymerase chain reaction and tissue immunostaining imply that C21orf63 is expressed on epithelia of various human tissues.
MeSH terms
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Cloning, Molecular
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Databases, Protein
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Epithelial Cells / metabolism
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Fluorescent Antibody Technique
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Galectins
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Gastric Mucosa / metabolism
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Gene Expression
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Glycosaminoglycans / metabolism
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Heparin / analogs & derivatives
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Heparin / metabolism*
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Heparitin Sulfate / metabolism
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Humans
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Immobilized Proteins / metabolism
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Lectins / chemistry
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Lectins / immunology
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Lectins / metabolism*
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Membrane Proteins / chemistry
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Membrane Proteins / immunology
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Membrane Proteins / metabolism*
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Microarray Analysis
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Microscopy, Fluorescence
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Mutant Proteins / chemistry
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Mutant Proteins / immunology
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Mutant Proteins / metabolism
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Organ Specificity
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Protein Binding
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Protein Structure, Tertiary*
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Stomach Neoplasms / metabolism
Substances
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EVA1C protein, human
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Galectins
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Glycosaminoglycans
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Immobilized Proteins
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Lectins
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Membrane Proteins
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Mutant Proteins
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Recombinant Fusion Proteins
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Heparin
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Heparitin Sulfate