Linear disulfide-containing low polymer as efficient DNA cleavage reagent

Bioorg Med Chem Lett. 2009 Jul 1;19(13):3458-60. doi: 10.1016/j.bmcl.2009.05.015. Epub 2009 May 9.

Abstract

A novel linear poly[1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane] (PBMAC) containing macrocyclic polyamine was synthesized through oxidation of 1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane (BMAC) and characterized. Gel electrophoresis experiments showed that PBMAC can promote the DNA cleavage more efficiently than its monomer under physiological conditions without any thiol additives. Fluorescence quenching and DNA melting experiments demonstrated that PBMAC has stronger binding ability with DNA than that of monomer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aza Compounds / chemical synthesis*
  • Aza Compounds / chemistry
  • Cycloparaffins / chemical synthesis*
  • Cycloparaffins / chemistry
  • DNA Cleavage / drug effects*
  • Disulfides / chemistry*
  • Fluorescent Dyes / chemistry
  • Polyamines / chemistry
  • Polymers / chemical synthesis
  • Polymers / chemistry*
  • Polymers / metabolism
  • Transition Temperature

Substances

  • Aza Compounds
  • Cycloparaffins
  • Disulfides
  • Fluorescent Dyes
  • Polyamines
  • Polymers
  • poly(1,7-bis(mercaptoacetyl)-1,4,7,10-tetraazacyclododecane)