Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis

J Clin Microbiol. 2009 Jul;47(7):2252-5. doi: 10.1128/JCM.00033-09. Epub 2009 May 20.

Abstract

A rapid assay for eubacterial species identification is described using high-resolution melt analysis to characterize PCR products. Unique melt profiles generated from multiple hypervariable regions of the 16S rRNA gene for 100 clinically relevant bacterial pathogens, including category A and B biothreat agents and their surrogates, allowed highly specific species identification.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacteria / isolation & purification*
  • Bacterial Infections / diagnosis*
  • Bioterrorism
  • DNA, Bacterial / genetics*
  • DNA, Ribosomal / genetics
  • Environmental Microbiology*
  • Humans
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 16S / genetics
  • Transition Temperature*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S