Overexpression of the CXCL3 gene in response to oxidized low-density lipoprotein is associated with the presence of tendon xanthomas in familial hypercholesterolemia

Biochem Cell Biol. 2009 Jun;87(3):493-8. doi: 10.1139/o09-006.

Abstract

To examine if overexpression of certain chemokines and proinflammatory cytokines in response to oxidized low-density lipoprotein could be involved in the onset and development of tendon xanthomas (TX), we quantified IL-1beta, TNF-alpha, and IL-8 and compared gene expression of PPAR-gamma, NF-kappaBIA, IL-8, IL-1beta, CXCL3, tryptase, and TNF-alpha in macrophages of familial hypercholesterolemia subjects with and without TX stimulated with oxidized low-density lipoprotein at 1, 3, 6, and 18 h of incubation. We propose that chemokines belonging to the CXC family could play an important role in the etiology of TX, with CXCL3 being a possible biological marker of onset and development of TX.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chemokines, CXC / genetics*
  • Connective Tissue Diseases / complications
  • Connective Tissue Diseases / genetics*
  • Female
  • Gene Expression*
  • Humans
  • Hyperlipoproteinemia Type II / complications
  • Hyperlipoproteinemia Type II / genetics*
  • Lipoproteins, LDL / physiology*
  • Male
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tendons / pathology*
  • Xanthomatosis / complications
  • Xanthomatosis / genetics*

Substances

  • CXCL3 protein, human
  • Chemokines, CXC
  • Lipoproteins, LDL
  • oxidized low density lipoprotein