Abstract
In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 microg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Camphanes
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Chromatography, Micellar Electrokinetic Capillary / instrumentation
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Chromatography, Micellar Electrokinetic Capillary / methods*
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Diterpenes / analysis*
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Diterpenes / chemistry
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Diterpenes / isolation & purification*
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Drugs, Chinese Herbal / chemistry
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Hydrogen-Ion Concentration
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Methanol / chemistry
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Molecular Structure
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Panax notoginseng
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Salvia / chemistry*
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Salvia / classification*
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Salvia miltiorrhiza
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Sodium Dodecyl Sulfate / chemistry
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Solvents / chemistry
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Time Factors
Substances
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Camphanes
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Diterpenes
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Drugs, Chinese Herbal
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Solvents
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danshen dripping pill
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Sodium Dodecyl Sulfate
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Methanol