Isolation and analysis of bioactive diterpenoids in Salvia species (Salvia chionantha and Salvia kronenburgiii) by micellar electrokinetic capillary chromatography

J Pharm Biomed Anal. 2010 Jan 20;51(2):439-42. doi: 10.1016/j.jpba.2009.04.012. Epub 2009 Apr 19.

Abstract

In the present work, we isolated two bioactive diterpenoids, horminone and 7-O-acetylhorminone and developed a micellar electrokinetic chromatography (MEKC) method for the simultaneous quantitative analysis of them in Turkish Salvia species. The optimal separation electrolyte was 50 mmol/L SDS and 25% methanol at pH 11.5. The limits of detection (S/N=3) were 3.269 and 4.518 microg/mL for horminone and 7-O-acetylhorminone, respectively. The method has been applied successfully to analyze these two components in Salvia chionantha and Salvia kronenburgii acetone extracts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Camphanes
  • Chromatography, Micellar Electrokinetic Capillary / instrumentation
  • Chromatography, Micellar Electrokinetic Capillary / methods*
  • Diterpenes / analysis*
  • Diterpenes / chemistry
  • Diterpenes / isolation & purification*
  • Drugs, Chinese Herbal / chemistry
  • Hydrogen-Ion Concentration
  • Methanol / chemistry
  • Molecular Structure
  • Panax notoginseng
  • Salvia / chemistry*
  • Salvia / classification*
  • Salvia miltiorrhiza
  • Sodium Dodecyl Sulfate / chemistry
  • Solvents / chemistry
  • Time Factors

Substances

  • Camphanes
  • Diterpenes
  • Drugs, Chinese Herbal
  • Solvents
  • danshen dripping pill
  • Sodium Dodecyl Sulfate
  • Methanol