A leukemic plasmacytoid dendritic cell line, PMDC05, with the ability to secrete IFN-alpha by stimulation via Toll-like receptors and present antigens to naïve T cells

Leuk Res. 2009 Sep;33(9):1224-32. doi: 10.1016/j.leukres.2009.03.047. Epub 2009 May 13.

Abstract

We established a plasmacytoid dendritic cell (pDC) line (PMDC05) from leukemia cells of pDC leukemia. PMDC05 cells were positive for CD4, CD56, CD33, HLA-DR, CD123 (IL-3Ralpha) and CD86 in the absence of lineage markers. mRNA of TLR1, TLR2, TLR4, TLR7 and TLR9 was clearly expressed and among these TLRs, TLR7 was prominent. Production of IFN-alpha and IL-12 in PMDC05 was enhanced by the stimulation with CpG-A and LPS, respectively. PMDC05 possessed a considerable antigen presenting ability, which was enhanced by culturing with IL3, influenza virus or LPS. PMDC05 could be a useful tool for investigating the pathophysiology of pDCL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / immunology
  • Base Sequence
  • Cell Line, Tumor
  • DNA Primers
  • Dendritic Cells / immunology
  • Dendritic Cells / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Interferon-alpha / metabolism*
  • Karyotyping
  • Leukemia, Plasma Cell / immunology
  • Leukemia, Plasma Cell / metabolism*
  • Leukemia, Plasma Cell / pathology
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / immunology*
  • Toll-Like Receptors / physiology*

Substances

  • Antigens
  • DNA Primers
  • Interferon-alpha
  • Toll-Like Receptors