Comparison of chromogenic media to BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR for detection of MRSA in nasal swabs

J Clin Microbiol. 2009 Jul;47(7):2281-3. doi: 10.1128/JCM.02256-08. Epub 2009 May 13.

Abstract

To select a method for detecting methicillin-resistant Staphylococcus aureus (MRSA) in nasal swabs, we compared BD GeneOhm MRSA PCR and various culture media (mannitol salt agar with cefoxitin, MRSASelect, CHROMagar MRSA, and Spectra MRSA). While PCR detection of MRSA was more rapid, MRSASelect and Spectra MRSA demonstrated performance equivalent to that of PCR with maximal detection at 24 h.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Carrier State / diagnosis*
  • Carrier State / microbiology
  • Culture Media / chemistry*
  • Humans
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification*
  • Nasal Mucosa / microbiology*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Time Factors

Substances

  • Culture Media