Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

Chem Commun (Camb). 2009 May 28:(20):2872-4. doi: 10.1039/b823057a. Epub 2009 Mar 31.

Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.

Publication types

  • Evaluation Study

MeSH terms

  • Biosensing Techniques / methods*
  • Calibration
  • Cross-Linking Reagents / chemistry
  • DNA / analysis*
  • DNA / chemistry
  • DNA / metabolism
  • Deoxyribonuclease I / metabolism
  • Fluorescent Dyes / analysis*
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Gold / chemistry
  • Hydrolysis
  • Lymphocyte Function-Associated Antigen-1 / analysis*
  • Lymphocyte Function-Associated Antigen-1 / chemistry
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Metal Nanoparticles / chemistry*
  • Silver / chemistry
  • Trypsin / metabolism

Substances

  • Cross-Linking Reagents
  • Fluorescent Dyes
  • Lymphocyte Function-Associated Antigen-1
  • Silver
  • Gold
  • DNA
  • Deoxyribonuclease I
  • Trypsin