Abstract
The ethanol extract of Pithecellobium clypearia Benth (PCE) was characterized to be rich in polyphenols by HPLC analysis, and investigated for its anti-inflammatory and anti-allergic activities. In our assay, PCE showed anti-inflammatory activity in both acute and auto-immune inflammation animal models. Administration of PCE can effectively inhibit the croton oil-induced ear edema and capillary permeability, the carrageenin-induced paw edema, and the liver injury caused by propionibacterium acnes plus lipopolysaccharide. PCE was also found to possess anti-allergic activity in inhibiting the DNFB-induced delayed hypersensitivity reaction. Meanwhile, seven main components (1-7) from PCE were studied for their effect on histamine release stimulated by compound 48/80 from rat peritoneal mast cells in vitro. Compound 2 ((-)-epigallocatechin-7-gallate), 3 ((-)-5, 7, 3', 4', 5'-pentahydroxyflavan), and 5 ((-)-tetra hydroxyflavan-7-gallate) showed significant inhibition effect on histamine release.
MeSH terms
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Animals
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Anti-Allergic Agents / isolation & purification
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Anti-Allergic Agents / pharmacology*
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Anti-Allergic Agents / therapeutic use
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Anti-Inflammatory Agents / isolation & purification
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Anti-Inflammatory Agents / pharmacology*
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Anti-Inflammatory Agents / therapeutic use
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Benzopyrans / isolation & purification
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Benzopyrans / pharmacology*
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Carrageenan
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Catechin / analogs & derivatives*
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Catechin / isolation & purification
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Catechin / pharmacology
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Croton Oil
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Disease Models, Animal
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Edema / drug therapy
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Fabaceae / chemistry*
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Histamine Antagonists / isolation & purification
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Histamine Antagonists / pharmacology
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Hypersensitivity / drug therapy
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Inflammation / drug therapy
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Lipopolysaccharides
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Liver / drug effects
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Male
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Mast Cells / drug effects
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Mice
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Molecular Structure
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Leaves
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Plant Stems
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Propionibacterium acnes
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Rats
Substances
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5,7,3',4',5'-pentahydroxyflavan
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Anti-Allergic Agents
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Anti-Inflammatory Agents
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Benzopyrans
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Histamine Antagonists
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Lipopolysaccharides
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Plant Extracts
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gallocatechin-7-gallate
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tetrahydroxyflavan-7-gallate
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Croton Oil
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Catechin
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Carrageenan