Abstract
The bioassay-guided fractionation of a MeOH extract of the heartwood of Caesalpinia sappan L. provided two neuroprotective compounds, sappanchalcone (2) and 4-O-methylepisappanol (3), together with a methoxychalcone, isoliquiritigenin 2'-methyl ether (1), and three aromatic compounds, 4-O-methylsappanol (4), caesalpine J (5), pluchoic acid (6). At concentrations of 20-40 microM, compound 2 showed significant cytoprotective effects against glutamate-induced oxidative stress through the induction of heme oxygenase (HO)-1 in HT22-immortalized hippocampal cells. Compound 3 also showed moderate neuroprotective effect at 40 microM, but compounds 1, 4-6 did not show any protective effects against glutamate-induced cytotoxicity in HT22 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Caesalpinia / chemistry*
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Cell Line
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Cell Nucleus / drug effects
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Cell Nucleus / enzymology
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Cell Nucleus / metabolism
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Cell Survival / drug effects
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Chalcone / analogs & derivatives*
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Chalcone / isolation & purification
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Chalcone / pharmacology
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Cytosol / drug effects
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Cytosol / enzymology
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Cytosol / metabolism
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Dose-Response Relationship, Drug
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Glutamic Acid / adverse effects*
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Heme Oxygenase-1 / biosynthesis
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Hippocampus / cytology
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Mice
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Molecular Structure
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Neuroprotective Agents / isolation & purification
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Neuroprotective Agents / pharmacology*
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Oxidative Stress / drug effects*
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Reactive Oxygen Species / metabolism
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Structure-Activity Relationship
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Wood
Substances
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Neuroprotective Agents
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Plant Extracts
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Reactive Oxygen Species
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isoliquiritigenin 2'-methyl ether
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Glutamic Acid
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Chalcone
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Heme Oxygenase-1