Cytoprotective constituents of the heartwood of Caesalpinia sappan on glutamate-induced oxidative damage in HT22 cells

Biol Pharm Bull. 2009 May;32(5):945-9. doi: 10.1248/bpb.32.945.

Abstract

The bioassay-guided fractionation of a MeOH extract of the heartwood of Caesalpinia sappan L. provided two neuroprotective compounds, sappanchalcone (2) and 4-O-methylepisappanol (3), together with a methoxychalcone, isoliquiritigenin 2'-methyl ether (1), and three aromatic compounds, 4-O-methylsappanol (4), caesalpine J (5), pluchoic acid (6). At concentrations of 20-40 microM, compound 2 showed significant cytoprotective effects against glutamate-induced oxidative stress through the induction of heme oxygenase (HO)-1 in HT22-immortalized hippocampal cells. Compound 3 also showed moderate neuroprotective effect at 40 microM, but compounds 1, 4-6 did not show any protective effects against glutamate-induced cytotoxicity in HT22 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Caesalpinia / chemistry*
  • Cell Line
  • Cell Nucleus / drug effects
  • Cell Nucleus / enzymology
  • Cell Nucleus / metabolism
  • Cell Survival / drug effects
  • Chalcone / analogs & derivatives*
  • Chalcone / isolation & purification
  • Chalcone / pharmacology
  • Cytosol / drug effects
  • Cytosol / enzymology
  • Cytosol / metabolism
  • Dose-Response Relationship, Drug
  • Glutamic Acid / adverse effects*
  • Heme Oxygenase-1 / biosynthesis
  • Hippocampus / cytology
  • Mice
  • Molecular Structure
  • Neuroprotective Agents / isolation & purification
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / drug effects*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Structure-Activity Relationship
  • Wood

Substances

  • Neuroprotective Agents
  • Plant Extracts
  • Reactive Oxygen Species
  • isoliquiritigenin 2'-methyl ether
  • Glutamic Acid
  • Chalcone
  • Heme Oxygenase-1