Scaffolded DNA origami of a DNA tetrahedron molecular container

Yonggang Ke; Jaswinder Sharma; Minghui Liu; Kasper Jahn; Yan Liu; Hao Yan

doi:10.1021/nl901165f

Scaffolded DNA origami of a DNA tetrahedron molecular container

Nano Lett. 2009 Jun;9(6):2445-7. doi: 10.1021/nl901165f.

Authors

Yonggang Ke¹, Jaswinder Sharma, Minghui Liu, Kasper Jahn, Yan Liu, Hao Yan

Affiliation

¹ Department of Chemistry and Biochemistry and The Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.

PMID: 19419184
DOI: 10.1021/nl901165f

Abstract

We describe a strategy of scaffolded DNA origami to design and construct 3D molecular cages of tetrahedron geometry with inside volume closed by triangular faces. Each edge of the triangular face is approximately 54 nm in dimension. The estimated total external volume and the internal cavity of the triangular pyramid are about 1.8 x 10(-23) and 1.5 x 10(-23) m(3), respectively. Correct formation of the tetrahedron DNA cage was verified by gel electrophoresis, atomic force microscopy, transmission electron microscopy, and dynamic light scattering techniques.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacteriophage M13 / chemistry
Bacteriophage M13 / genetics
DNA, Single-Stranded / chemistry*
DNA, Single-Stranded / ultrastructure
Electrophoresis
Light
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Nanostructures / chemistry*
Nanostructures / ultrastructure
Nucleic Acid Conformation*
Scattering, Radiation

Substances

DNA, Single-Stranded