Characteristics of human and mouse orthologous gene sequences which have large G+C content variations were investigated in this study. The orthologous gene pairs were classified into two groups according to the deviation between human and mouse G+C content at the third codon position (GC3) and were subsequently analyzed. In one group, mouse genes had higher GC3 than the corresponding human genes and in another group, human genes had higher GC3 than mouse. Furthermore, the orthologous pairs were separated based on the deviation between human or mouse GC3 and the G+C content at the third codon position of identical codons (IC3), to examine the effect of increased or decreased G+C content in human or mouse sequences. The nucleotide substitution patterns between human and mouse sequences in the two groups were remarkably distinct, and consistent with the state of G+C-rich or G+C-poor sequences. The effect of increase or decrease of G+C content in human or mouse sequences was not clear in the nucleotide substitution patterns. The chromosomal locations of human and mouse orthologous gene pairs were different between the two groups. The genes located on an identical syntenic segment showed the trend of having similar G+C content. Moreover, the same gene order of some genes on different chromosomes of both species demonstrated the gene rearrangements between human and mouse. Our study indicated that the chromosomal locations and rearrangements are associated with the GC3 variation between human and mouse sequences.
Keywords: G+C content variation; Human mouse orthologs; gene location; gene rearrangement.; nucleotide substitution.