Abstract
The immunopharmacological activities of beta-glucans with a backbone of beta-1,3/beta-1,6-linkages associated with anti-tumor, anti-viral, bacterial and fungal infections have been well documented. Dectin-1, a specific pattern recognition receptor for beta-1,3/beta-1,6-glucans, is expressed mainly on phagocytes, especially macrophages and dendritic cells (DCs). In this study, the encoding nucleotide for the carbohydrate-recognition domain (CRD) of porcine dectin-1 was sequenced for the first time, and the immunomodulatory functions of a synthetic particulate beta-glucan (p-beta-glucan) were examined. Results showed that p-beta-glucan significantly enhanced cell activity and phagocytosis in porcine alveolar macrophages (AMs), immature DCs (imDCs) and mature DCs (mDCs), in a similar way to zymosan. Zymosan enhanced dectin-1/TLR2/TLR4 expression and TNF-alpha/IL-10 production in all of three types of cell, whereas p-beta-glucan increased dectin-1/TLR4 and TNF-alpha/IL-12 production in AMs but inhibited IL-10 in mDCs. These results indicate that the complex collaborating interactions between dectin-1 and TLRs in the recognition of beta-1,3/beta-1,6-glucans with different structural features may direct different cellular responses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Bone Marrow Cells / cytology
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / immunology
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Cell Differentiation
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Cloning, Molecular
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DNA Primers / genetics
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Dendritic Cells / cytology
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Dendritic Cells / drug effects*
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Dendritic Cells / immunology*
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Host-Pathogen Interactions / immunology
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Humans
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Immunologic Factors / chemistry
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Immunologic Factors / immunology
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Immunologic Factors / pharmacology*
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In Vitro Techniques
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Interleukin-10 / biosynthesis
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Interleukin-12 / biosynthesis
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Lectins, C-Type
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Macromolecular Substances / chemistry
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Macromolecular Substances / immunology
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Macromolecular Substances / pharmacology
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Macrophages, Alveolar / cytology
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Macrophages, Alveolar / drug effects*
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Macrophages, Alveolar / immunology*
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Male
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Mice
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Microscopy, Electron, Transmission
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Molecular Sequence Data
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / immunology
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Nerve Tissue Proteins / metabolism
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Phagocytosis
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Protein Structure, Tertiary
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Sequence Homology, Nucleic Acid
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Species Specificity
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Sus scrofa / genetics
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Sus scrofa / immunology*
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Toll-Like Receptor 2 / metabolism
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Toll-Like Receptor 4 / metabolism
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Tumor Necrosis Factor-alpha / biosynthesis
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beta-Glucans / chemistry
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beta-Glucans / immunology
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beta-Glucans / pharmacology*
Substances
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DNA Primers
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Immunologic Factors
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Lectins, C-Type
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Macromolecular Substances
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Membrane Proteins
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Nerve Tissue Proteins
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Toll-Like Receptor 2
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Toll-Like Receptor 4
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Tumor Necrosis Factor-alpha
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beta-Glucans
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dectin 1
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Interleukin-10
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Interleukin-12