Abstract
The eukaryotic genome forms a chromatin structure that contains repeating nucleosome structures. Nucleosome packaging is regulated by chromatin remodeling factors such as histone chaperones. The Saccharomyces cerevisiae H3/H4 histone chaperones, CAF-1 and Asf1, regulate DNA replication and chromatin assembly. CAF-1 function is largely restricted to non-transcriptional processes in heterochromatin, whereas Asf1 regulates transcription together with another H3/H4 chaperone, HIR. This study examined the role of the yeast H3/H4 histone chaperones, Asf1, HIR, and CAF-1 in chromatin dynamics during transcription. Unexpectedly, CAF-1 was recruited to the actively transcribed region in a similar way to HIR and Asf1. In addition, the three histone chaperones genetically interacted with Set2-dependent H3 K36 methylation. Similar to histone chaperones, Set2 was required for tolerance to excess histone H3 but not to excess H2A, suggesting that CAF-1, Asf1, HIR, and Set2 function in a related pathway and target chromatin during transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism
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Cell Cycle Proteins / physiology
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Histones / metabolism
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Methyltransferases / genetics
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Methyltransferases / metabolism
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Methyltransferases / physiology
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism
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Molecular Chaperones / physiology*
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Protein Kinases / physiology
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Ribonucleases / genetics
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Ribonucleases / metabolism
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Ribonucleases / physiology*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Saccharomyces cerevisiae Proteins / physiology*
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TATA-Box Binding Protein / genetics
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TATA-Box Binding Protein / metabolism
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TATA-Box Binding Protein / physiology
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Transcription, Genetic / genetics*
Substances
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ASF1 protein, S cerevisiae
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CTDK-I protein complex, S cerevisiae
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Cell Cycle Proteins
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Histones
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Molecular Chaperones
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RTF1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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TATA-Box Binding Protein
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Methyltransferases
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Set2 protein, S cerevisiae
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Protein Kinases
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Ribonucleases
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POP2 protein, S cerevisiae