Structural determination of the O-antigenic polysaccharide from Salmonella Mara (O:39)

Jerzy Gajdus; Zbigniew Kaczyński; Joanna Smietana; Piotr Stepnowski

doi:10.1016/j.carres.2009.02.015

Structural determination of the O-antigenic polysaccharide from Salmonella Mara (O:39)

Carbohydr Res. 2009 May 26;344(8):1054-7. doi: 10.1016/j.carres.2009.02.015. Epub 2009 Feb 25.

Authors

Jerzy Gajdus¹, Zbigniew Kaczyński, Joanna Smietana, Piotr Stepnowski

Affiliation

¹ Faculty of Chemistry, University of Gdańsk, Gdańsk, Poland.

PMID: 19394591
DOI: 10.1016/j.carres.2009.02.015

Abstract

The O-antigenic polysaccharide of Salmonella Mara O:39 (formerly Q) was investigated by sugar and methylation analyses, absolute configuration assignment, mass spectrometry and NMR spectroscopy. The experiments revealed an O-polysaccharide chain composed of the following linear tetrasaccharide repeating units with the structure: -->2)-alpha-L-Quip3NAc-(1-->3)-alpha-D-Manp-(1-->3)-alpha-L-Fucp-(1-->3)-alpha-D-GalpNAc-(1--> where alpha-L-Quip3NAc is the residue of 3-acetamido-3,6-dideoxy-alpha-L-glucopyranose. This repeating unit is the first published structure of the O-polysaccharide from 27 serotypes of Salmonella bacteria belonging to serogroup O:39 in the Kauffmann-White classification system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Carbohydrates
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
O Antigens / chemistry*
Salmonella / chemistry*

Substances

Carbohydrates
O Antigens