Abstract
ACI is the most widely used cell-based surgical procedure for the repair of articular cartilage defects. The method is based on in vitro chondrocyte cultivation. Two different culture conditions, rotating-wall-vessel bioreactor and static culture, were assessed by their effect on the re-differentiation potential of human articular chondrocytes seeded into a hydrogel scaffold. Gene expression analysis of the tissue-engineered construct revealed no significant difference between the tested systems.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aggrecans / metabolism
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Bioreactors
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Cartilage, Articular / cytology*
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Cartilage, Articular / metabolism
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Cell Culture Techniques
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Cell Differentiation* / genetics
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Cells, Cultured
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Chondrocytes / cytology*
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Chondrocytes / metabolism
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Chondrocytes / transplantation
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Collagen Type I / metabolism
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Collagen Type II / metabolism
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Gene Expression
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Tissue Engineering / methods*
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Tissue Scaffolds
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Versicans / metabolism
Substances
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Aggrecans
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Collagen Type I
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Collagen Type II
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Versicans
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Hydrogel, Polyethylene Glycol Dimethacrylate