Re-differentiation of human articular chondrocytes is not enhanced by a rotary bioreactor system

Folia Biol (Praha). 2008;54(6):177-9.

Abstract

ACI is the most widely used cell-based surgical procedure for the repair of articular cartilage defects. The method is based on in vitro chondrocyte cultivation. Two different culture conditions, rotating-wall-vessel bioreactor and static culture, were assessed by their effect on the re-differentiation potential of human articular chondrocytes seeded into a hydrogel scaffold. Gene expression analysis of the tissue-engineered construct revealed no significant difference between the tested systems.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggrecans / metabolism
  • Bioreactors
  • Cartilage, Articular / cytology*
  • Cartilage, Articular / metabolism
  • Cell Culture Techniques
  • Cell Differentiation* / genetics
  • Cells, Cultured
  • Chondrocytes / cytology*
  • Chondrocytes / metabolism
  • Chondrocytes / transplantation
  • Collagen Type I / metabolism
  • Collagen Type II / metabolism
  • Gene Expression
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Tissue Engineering / methods*
  • Tissue Scaffolds
  • Versicans / metabolism

Substances

  • Aggrecans
  • Collagen Type I
  • Collagen Type II
  • Versicans
  • Hydrogel, Polyethylene Glycol Dimethacrylate