Taking advantage of nonspecific trypsin cleavages for the identification of seed storage proteins in cereals

J Proteome Res. 2009 Jun;8(6):3182-90. doi: 10.1021/pr801093f.

Abstract

The lack of basic amino acids in seed storage proteins has resulted in the proposal to use chymotrypsin in their study. A comparative study of trypsin and chymotrypsin digestion initially confirmed this preference; however, reanalysis of the trypsin data set defining the specificity as 'semitrypsin' provided enough extra data to bridge the gap between both proteases. Rationale as to why numerous semitryptic peptides are observed in the study of these proteins is provided.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chymotrypsin / metabolism*
  • Computer Simulation
  • Electrophoresis, Gel, Two-Dimensional
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Peptide Fragments / metabolism
  • Proteomics / methods
  • Seed Storage Proteins / chemistry*
  • Seed Storage Proteins / metabolism
  • Seeds / chemistry*
  • Trypsin / metabolism*
  • Zea mays / chemistry*
  • Zea mays / metabolism

Substances

  • Peptide Fragments
  • Seed Storage Proteins
  • Chymotrypsin
  • Trypsin