Cordycepin inhibits UVB-induced matrix metalloproteinase expression by suppressing the NF-kappaB pathway in human dermal fibroblasts

Exp Mol Med. 2009 Aug 31;41(8):548-54. doi: 10.3858/emm.2009.41.8.060.

Abstract

Cordycepin (3-deoxyadenosine) has been shown to exhibit many pharmacological activities, including anti-cancer, anti-inflammatory, and anti-infection activities. However, the anti-skin photoaging effects of cordycepin have not yet been reported. In the present study, we investigated the inhibitory effects of cordycepin on matrix metalloproteinase-1 (MMP-1) and -3 expressions of the human dermal fibroblast cells. Western blot analysis and real-time PCR revealed cordycepin inhibited UVB-induced MMP-1 and -3 expressions in a dose-dependent manner. UVB strongly activated NF-kappaB activity, which was determined by IkappaBalpha degradation, nuclear localization of p50 and p65 subunit, and NF-kappaB binding activity. However, UVB-induced NF-kappaB activation and MMP expression were completely blocked by cordycepin pretreatment. These findings suggest that cordycepin could prevent UVB-induced MMPs expressions through inhibition of NF-kappaB activation. In conclusion, cordycepin might be used as a potential agent for the prevention and treatment of skin photoaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology
  • Cells, Cultured
  • Deoxyadenosines / pharmacology*
  • Dermis* / cytology
  • Dermis* / drug effects
  • Dermis* / physiology
  • Dermis* / radiation effects
  • Dose-Response Relationship, Drug
  • Enzyme Induction / drug effects
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Fibroblasts / radiation effects
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Infant, Newborn
  • Male
  • Matrix Metalloproteinase 1* / biosynthesis
  • Matrix Metalloproteinase 1* / genetics
  • Matrix Metalloproteinase 1* / radiation effects
  • Matrix Metalloproteinase 3 / biosynthesis*
  • Matrix Metalloproteinase 3 / genetics
  • Matrix Metalloproteinase 3 / radiation effects
  • Matrix Metalloproteinase Inhibitors
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / genetics
  • NF-kappa B / metabolism
  • Skin / physiopathology
  • Skin / radiation effects
  • Ultraviolet Rays*

Substances

  • Deoxyadenosines
  • Matrix Metalloproteinase Inhibitors
  • NF-kappa B
  • MMP3 protein, human
  • Matrix Metalloproteinase 3
  • MMP1 protein, human
  • Matrix Metalloproteinase 1
  • cordycepin