Electrophoretic mobility shift assays for the analysis of DNA-protein interactions

Manon Gaudreault; Marie-Eve Gingras; Maryse Lessard; Steeve Leclerc; Sylvain L Guérin

doi:10.1007/978-1-60327-015-1_2

Electrophoretic mobility shift assays for the analysis of DNA-protein interactions

Methods Mol Biol. 2009:543:15-35. doi: 10.1007/978-1-60327-015-1_2.

Authors

Manon Gaudreault¹, Marie-Eve Gingras, Maryse Lessard, Steeve Leclerc, Sylvain L Guérin

Affiliation

¹ Oncology and Molecular Endocrinology Research Center, CHUL, Centre Hospitalier Universitaire de Québec and Laval University, 2705 Laurier Blvd, Québec, QC, Canada, G1V 4G2.

PMID: 19378156
DOI: 10.1007/978-1-60327-015-1_2

Abstract

Electromobility shift assay is a simple, efficient, and rapid method for the study of specific DNA-protein interactions. It relies on the reduction in the electrophoretic mobility conferred to a DNA fragment by an interacting protein. The technique is suitable to qualitative, quantitative, and kinetic analyses. It can also be used to analyze conformational changes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / metabolism*
DNA Probes / isolation & purification
Electrophoretic Mobility Shift Assay / methods*
Isotope Labeling
Nuclear Proteins / metabolism
Protein Binding / drug effects
Proteins / metabolism*
Sodium Chloride / pharmacology

Substances

DNA Probes
Nuclear Proteins
Proteins
Sodium Chloride
DNA