Use of non-radioactive detection method for north- and southwestern blot

Methods Mol Biol. 2009:536:441-9. doi: 10.1007/978-1-59745-542-8_44.

Abstract

Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).

MeSH terms

  • Autoantigens / chemistry
  • Autoantigens / metabolism
  • Blotting, Southwestern / instrumentation
  • Blotting, Southwestern / methods*
  • DNA / chemistry
  • DNA / metabolism*
  • DNA Probes / chemistry
  • DNA Probes / metabolism
  • Digoxigenin / chemistry
  • Humans
  • Protein Binding
  • Proteins / chemistry
  • Proteins / metabolism*
  • RNA / chemistry
  • RNA / metabolism*
  • RNA Probes / chemistry
  • RNA Probes / metabolism
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / metabolism
  • SS-B Antigen

Substances

  • Autoantigens
  • DNA Probes
  • Proteins
  • RNA Probes
  • Ribonucleoproteins
  • RNA
  • DNA
  • Digoxigenin