Abstract
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here, we describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
MeSH terms
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Autoantigens / chemistry
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Autoantigens / metabolism
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Blotting, Southwestern / instrumentation
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Blotting, Southwestern / methods*
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DNA / chemistry
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DNA / metabolism*
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DNA Probes / chemistry
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DNA Probes / metabolism
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Digoxigenin / chemistry
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Humans
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Protein Binding
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Proteins / chemistry
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Proteins / metabolism*
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RNA / chemistry
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RNA / metabolism*
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RNA Probes / chemistry
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RNA Probes / metabolism
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Ribonucleoproteins / chemistry
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Ribonucleoproteins / metabolism
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SS-B Antigen
Substances
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Autoantigens
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DNA Probes
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Proteins
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RNA Probes
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Ribonucleoproteins
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RNA
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DNA
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Digoxigenin