A miniaturized blotting system for simultaneous detecting of different autoantibodies

Ulrich Canzler; Holger Bartsch; Kai Grossmann; Werner Lehmann; Karsten Conrad; Biji T Kurien; Yaser Dorri; R Hal Scofield; Michael Bachmann

doi:10.1007/978-1-59745-542-8_15

A miniaturized blotting system for simultaneous detecting of different autoantibodies

Methods Mol Biol. 2009:536:129-37. doi: 10.1007/978-1-59745-542-8_15.

Authors

Ulrich Canzler¹, Holger Bartsch, Kai Grossmann, Werner Lehmann, Karsten Conrad, Biji T Kurien, Yaser Dorri, R Hal Scofield, Michael Bachmann

Affiliation

¹ Institut für Immunologie, Carl Gustav Carus Technische Universität Dresden, Fetscherstrasse 74, 01307, Dresden, Germany.

PMID: 19378052
DOI: 10.1007/978-1-59745-542-8_15

Abstract

Sera of tumor patients frequently contain autoantibodies to tumor-associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autoantibodies / genetics
Autoantibodies / immunology*
Autoantigens / immunology
Humans
Immunoblotting* / instrumentation
Immunoblotting* / methods
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology

Substances

Autoantibodies
Autoantigens
Recombinant Fusion Proteins