Abstract
We used translation-blocking morpholinos to reduce protein levels in Giardia intestinalis. Twenty-four hours after electroporation with morpholinos targeting either green fluorescent protein or kinesin-2b, levels of these proteins were reduced by 60%. An epitope-tagged transgene can also be used as a reporter for morpholino efficacy with targets lacking specific antibodies.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Gene Knockdown Techniques
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Gene Silencing*
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Giardia lamblia / genetics*
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Giardia lamblia / metabolism
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Oligonucleotides, Antisense / genetics*
Substances
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Oligonucleotides, Antisense
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Green Fluorescent Proteins