Fabrication of a novel layer-by-layer film based glucose biosensor with compact arrangement of multi-components and glucose oxidase

Biosens Bioelectron. 2009 Jun 15;24(10):3131-4. doi: 10.1016/j.bios.2009.03.013. Epub 2009 Mar 19.

Abstract

Layer-by-layer (LbL) film based glucose biosensor was fabricated with alternative layers of a nanocomposite (comprising of multiwalled carbon nanotubes (MWNTs), Au nanoparticles (Au NPs) and thiol functionalized polyaniline (PANI(SH)) and glucose oxidase (GOx). The successful formation of multilayers was confirmed by UV-visible spectroscopy. The components in the nanocomposite provide adequate electron transfer path between GOx and the electrode. A high value for the rate constant of electron transfer process (27.84 s(-1)) was observed at [GOx/Au-(SH)PANI-g-MWNT](n)/ITO electrode. The [GOx/Au-(SH)PANI-g-MWNT](n) biosensor exhibited high sensitivity (3.97 microA/mM) for the detection of glucose over a concentration range of 1-9 mM with a low detection limit of 0.06 microM.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Enzymes, Immobilized
  • Equipment Design
  • Glucose / analysis*
  • Glucose Oxidase*
  • Gold
  • Metal Nanoparticles
  • Nanocomposites*
  • Nanotubes, Carbon
  • Static Electricity

Substances

  • Aniline Compounds
  • Enzymes, Immobilized
  • Nanotubes, Carbon
  • polyaniline
  • Gold
  • Glucose Oxidase
  • Glucose