Abstract
We developed an efficient cell culture system for genotype 4 hepatitis E virus using the HE-JF5/15F strain recovered from a fulminant hepatitis patient. The sixth-passage virus in the culture supernatant reached 1.5 x 10(8) copies/ml at 10 days postinoculation and possessed 10 nucleotide mutations with four amino acid changes.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution / genetics
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Cell Culture Techniques / methods
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Cell Line
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Genotype
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Hepatitis E / virology*
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Hepatitis E virus / genetics
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Hepatitis E virus / growth & development*
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Hepatitis E virus / isolation & purification*
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Mutation, Missense
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RNA, Viral / genetics
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Sequence Analysis, DNA