Fast detection of bacterial growth by using Portable Microbe Enrichment Unit (PMEU) and ChemPro100i((R)) gas sensor

Elias Hakalehto; Jouni Pesola; Anneli Heitto; Brajesh Bhanj Deo; Kari Rissanen; Ulla Sankilampi; Tarmo Humppi; Heikki Paakkanen

doi:10.1016/j.pathophys.2009.03.001

Fast detection of bacterial growth by using Portable Microbe Enrichment Unit (PMEU) and ChemPro100i((R)) gas sensor

Pathophysiology. 2009 Jun;16(1):57-62. doi: 10.1016/j.pathophys.2009.03.001. Epub 2009 Apr 14.

Authors

Elias Hakalehto¹, Jouni Pesola, Anneli Heitto, Brajesh Bhanj Deo, Kari Rissanen, Ulla Sankilampi, Tarmo Humppi, Heikki Paakkanen

Affiliation

¹ Department of Biosciences, University of Kuopio, P.O.B. 1627, FI-70211 Kuopio, Finland. elias.hakalehto@pp.inet.fi

PMID: 19369043
DOI: 10.1016/j.pathophys.2009.03.001

Abstract

The combination of the Portable Microbe Enrichment Unit (PMEU) cultivation and ChemPro100i((R)) Chemical Detector helps the fast detection of hospital infections. The first alarm of the microbe metabolism and growth in the enrichment culture was achieved in 2-3h when pure cultures of strains isolated from neonatal patients were tested. For the detection of minor concentrations of the bacteria, Bacillus, Staphylococcus, Klebsiella and Escherichia strains were diluted to 0.5-600cells per ml. Their preliminary detection was achieved within 4-5h. The combination of enrichment culture (PMEU) and detection of bacterial products by ion mobility spectrometer is a rapid and sensitive method for diagnosis of bacterial growth. This finding warrants further studies with clinical samples.