Rapid sequencing gel electrophoresis using glycerol-tolerant sodium taurine medium

Ken Hirano; Tomomi Ishido; Mitsuru Ishikawa

doi:10.1016/j.ab.2009.04.006

Rapid sequencing gel electrophoresis using glycerol-tolerant sodium taurine medium

Anal Biochem. 2009 Jul 1;390(1):100-1. doi: 10.1016/j.ab.2009.04.006. Epub 2009 Apr 11.

Authors

Ken Hirano¹, Tomomi Ishido, Mitsuru Ishikawa

Affiliation

¹ Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Takamatsu, Kagawa 761-0395, Japan. hirano-ken@aist.go.jp

PMID: 19364487
DOI: 10.1016/j.ab.2009.04.006

Abstract

We describe a new glycerol-tolerant sodium taurine (ST) medium for rapid sequencing gel electrophoresis by substituting the standard conductive media of Tris-boric acid-ethylenediaminetetraacetic acid (EDTA) (TBE) and Tris-taurine-EDTA (TTE) and other low-ionic-strength media of sodium boric acid (SB). Low-ionic-strength and cost-effective ST media gave glycerol tolerance up to 50% (v/v) glycerol-containing DNA sample solution, shorter running time, and better resolution to separate small DNA oligonucleotides (20-45 mer) in 12% denaturing sequencing gel electrophoresis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Polyacrylamide Gel / methods*
Glycerol / chemistry*
Oligonucleotides / analysis
Sequence Analysis, DNA / methods*
Taurine / chemistry*

Substances

Oligonucleotides
Taurine
Glycerol