Direct automatic determination of free and total anesthetic drugs in human plasma by use of a dual (microdialysis-microextraction by packed sorbent) sample treatment coupled at-line to NACE-MS

Gabriel Morales-Cid; Soledad Cárdenas; Bartolomé M Simonet; Miguel Valcárcel

doi:10.1002/elps.200800493

Direct automatic determination of free and total anesthetic drugs in human plasma by use of a dual (microdialysis-microextraction by packed sorbent) sample treatment coupled at-line to NACE-MS

Electrophoresis. 2009 May;30(10):1684-91. doi: 10.1002/elps.200800493.

Authors

Gabriel Morales-Cid¹, Soledad Cárdenas, Bartolomé M Simonet, Miguel Valcárcel

Affiliation

¹ Department of Analytical Chemistry, Campus de Rabanales, University of Cordoba, Cordoba, Spain.

PMID: 19360775
DOI: 10.1002/elps.200800493

Abstract

This paper reports for the first time the use of microextraction by packed sorbent in combination with CE. The combined system was used to determine anesthetic drugs in human plasma. A microdialysis fiber was coupled on-line to the microextraction unit in order to distinguish between free and total concentrations of drugs. The system was automated by connecting the microextraction unit to a syringe pump and interfacing it to a computer. The ensuing method allows the determination of 10 microg/L concentrations of free drugs and 1 microg/L concentrations of total drugs from only 200 microL of sample with an RSD of less than 9%.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anesthetics / blood*
Anesthetics / isolation & purification
Electrophoresis, Capillary / methods*
Humans
Mass Spectrometry / methods*
Microdialysis / methods*
Solid Phase Extraction / methods*

Substances

Anesthetics