Abstract
Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.
Publication types
-
Evaluation Study
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Escherichia coli / genetics
-
Escherichia coli / isolation & purification*
-
Escherichia coli Infections / diagnosis*
-
Humans
-
Infant
-
Polymerase Chain Reaction / economics
-
Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity