Aim: To investigate the effect of the activated LXR on the expression of IRAK-4 and NF-kappaB in the inflammatory response which was induced by LPS in Kupffer cells.
Methods: The Kuppfer cells were isolated from male Kunming mice by collagen perfusion in situ. These cells were divided into four groups: normal control group, LPS treatment group, LXR agonist T0901317 treatment group, LPS and T0901317 combined treatment group. The expression of LXR, IRAK-4 and NF-kappaB gene and their protein levels were detected by real time-PCR and Western blot, and the activity of NF-kappaB was observed by EMSA.
Results: The levels of LXR mRNA and protein reached the highest in T0901317 group but the lowest in LPS group. The levels of IRAK4 and NF-kappaB mRNA and protein in the combined-treatment group were evidently lower than those in LPS group. The activity of NF-kappaB reached the highest in LPS group but it was lower in the combined-treatment group and T0901317 group.
Conclusion: LXR agonists can effectively up-regulate the level of LXR gene and protein and down-regulate the levels of IRAK4 and NF-kappaB mRNA and protein expression. They can also inhibit activity of NF-kappaB.