One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae

Ratana Lawung; Rungrot Cherdtrakulkiat; Angkana Charoenwatanachokchai; Sunanta Nabu; Wanvisa Suksaluk; Virapong Prachayasittikul

doi:10.1016/j.mimet.2009.03.009

One-step PCR for the identification of multiple antimicrobial resistance in Neisseria gonorrhoeae

J Microbiol Methods. 2009 Jun;77(3):323-5. doi: 10.1016/j.mimet.2009.03.009. Epub 2009 Mar 31.

Authors

Ratana Lawung¹, Rungrot Cherdtrakulkiat, Angkana Charoenwatanachokchai, Sunanta Nabu, Wanvisa Suksaluk, Virapong Prachayasittikul

Affiliation

¹ Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

PMID: 19341768
DOI: 10.1016/j.mimet.2009.03.009

Abstract

One-step multiplex PCR was developed for the identification of gonococci and antimicrobial-resistant profiles. From forty Neisseria gonorrhoeae isolates, the penicillinase-producing N. gonorrhoeae (PPNG), the high-level tetracycline-resistant N. gonorrhoeae (TRNG), and the ciprofloxacin-resistant N. gonorrhoeae (CRNG) were successfully classified. Our method provides expediency and benefit to epidemiology and antimicrobial-resistance mobility with 100% sensitivity and specificity for gonococcal-detection. The detection limit was 500 CFU/reaction.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Drug Resistance, Bacterial*
Gonorrhea / microbiology*
Humans
Neisseria gonorrhoeae / drug effects*
Neisseria gonorrhoeae / genetics
Neisseria gonorrhoeae / isolation & purification
Neisseria gonorrhoeae / metabolism
Polymerase Chain Reaction / methods*
Sensitivity and Specificity

Substances

Anti-Bacterial Agents
Bacterial Proteins