Towards direct monitoring of discrete events in a catalytic cycle at the single molecule level

Rob Ameloot; Maarten Roeffaers; Mukulesh Baruah; Gert De Cremer; Bert Sels; Dirk De Vos; Johan Hofkens

doi:10.1039/b821657f

Towards direct monitoring of discrete events in a catalytic cycle at the single molecule level

Photochem Photobiol Sci. 2009 Apr;8(4):453-6. doi: 10.1039/b821657f. Epub 2009 Jan 22.

Authors

Rob Ameloot¹, Maarten Roeffaers, Mukulesh Baruah, Gert De Cremer, Bert Sels, Dirk De Vos, Johan Hofkens

Affiliation

¹ Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B-3001, Leuven, Belgium.

PMID: 19337657
DOI: 10.1039/b821657f

Abstract

The binding state dependent quenching behaviour of functionalized dyes opens perspectives on directly monitoring binding and dissociation events in a catalytic cycle at the single molecule level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Catalysis*
Fluorescence
Microscopy, Fluorescence / methods
Molecular Biology / methods*
Monitoring, Physiologic / methods
Sensitivity and Specificity