Abstract
Protein patterns of 129 Helicobacter pylori strains isolated from Korean and Colombian patients suffering from duodenal ulcer or gastric cancer were analyzed by the high-throughput methodology SELDI-TOF-MS. Eighteen statistically significant candidate biomarkers discriminating between the two clinical outcomes were selected by using the Mann-Whitney test. Three biomarker proteins were purified and identified as a neutrophil-activating protein NapA (HU HPAG1_0821), a RNA-binding protein (HPAG1_0813), and a DNA-binding histone-like protein HU, respectively (jhp0228). These novel biomarkers can be used for development of diagnostic assays predicting the evolution to gastric cancer in H. pylori-infected patients.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Biomarkers
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Chromatography, Ion Exchange
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Colombia
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Duodenal Ulcer / diagnosis*
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Duodenal Ulcer / metabolism
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Duodenal Ulcer / microbiology
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Helicobacter Infections / diagnosis*
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Helicobacter Infections / metabolism
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Helicobacter Infections / microbiology
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Helicobacter pylori / chemistry
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Helicobacter pylori / genetics
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Helicobacter pylori / metabolism*
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Korea
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Mass Spectrometry
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Molecular Sequence Data
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Protein Array Analysis
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Proteomics
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Reproducibility of Results
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Stomach Neoplasms / diagnosis*
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Stomach Neoplasms / metabolism
Substances
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Bacterial Proteins
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Biomarkers