Quantification of phenolic constituents and antioxidant activity of Pterodon emarginatus vogel seeds

Rafael C Dutra; Magda N Leite; Nádia R Barbosa

doi:10.3390/ijms9040606

Quantification of phenolic constituents and antioxidant activity of Pterodon emarginatus vogel seeds

Int J Mol Sci. 2008 Apr;9(4):606-614. doi: 10.3390/ijms9040606. Epub 2008 Apr 18.

Authors

Rafael C Dutra¹, Magda N Leite², Nádia R Barbosa¹

Affiliations

¹ Departamento de Alimentos e Toxicologia, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brasil.
² Departamento Farmacêutico, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, Martelos, CEP 36036-330, Juiz de Fora, MG, Brasil.

Abstract

In the present study the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents and the antioxidant activity of Pterodon emarginatus seeds were investigated in several samples prepared with different extraction procedures: essential oil (EO) using a Clevenger-type apparatus; hexanic (HF), ethyl acetate (EAF), buthanolic (BF) and methanolic (MF) fractions using Soxhlet extraction, and extracts (1 g/extract) obtained from different methods: reflux 80 degrees C/30 min, ultrasound/30 min, static maceration/48 h and heating plate 100 degrees C/45 min. These extracts were prepared using water or ethanol/water at 30:70 v/v, 50:50 v/v or 70:30 v/v. Antioxidant activity [2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH)] was tested only in the fractions obtained from Soxhlet extraction. The extract obtained from reflux using ethanol/water (70:30, v/v) showed the highest phenolic constituents level. The EAF, BF and MF showed DPPH scavenging activities with IC(50)=163.22, 18.89 and 10.15 microg/ml, respectively.

Keywords: DPPH; Leguminosae; Pterodon emarginatus; phenolic constituents.