Investigation of residual DNAs in sugar from sugar beet (Beta vulgaris L.)

Taichi Oguchi; Mari Onishi; Yukie Chikagawa; Takashi Kodama; Emiri Suzuki; Masaki Kasahara; Hiroshi Akiyama; Reiko Teshima; Satoshi Futo; Akihiro Hino; Satoshi Furui; Kazumi Kitta

doi:10.3358/shokueishi.50.41

Investigation of residual DNAs in sugar from sugar beet (Beta vulgaris L.)

Shokuhin Eiseigaku Zasshi. 2009 Feb;50(1):41-6. doi: 10.3358/shokueishi.50.41.

Authors

Taichi Oguchi¹, Mari Onishi, Yukie Chikagawa, Takashi Kodama, Emiri Suzuki, Masaki Kasahara, Hiroshi Akiyama, Reiko Teshima, Satoshi Futo, Akihiro Hino, Satoshi Furui, Kazumi Kitta

Affiliation

¹ National Agriculture and Food Research Organization, National Food Research Institute, Tsukuba, Ibaraki 3058642, Japan.

PMID: 19325225
DOI: 10.3358/shokueishi.50.41

Abstract

Genetically modified (GM) sugar beets have been bred for use as food and animal feed. To evaluate the applicability of GMO analyses to beet sugar products, we investigated residual DNA in eight sorts of in-process beet sugar samples and commercial beet sugar products. Polymerase chain reaction (PCR) analyses with taxon-specific primers indicated that sugar beet DNA was degraded at an early stage of sugar processing, and no PCR amplification was detected from the investigated sugar products because of low DNA recovery and/or PCR inhibition.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crops, Agricultural / chemistry
DNA, Plant / analysis*
Plants, Genetically Modified / genetics*
Polymerase Chain Reaction

Substances

DNA, Plant