Molecular genetics analysis for co-infection of Brugia malayi and Brugia pahangi in cat reservoirs based on internal transcribed spacer region 1

Southeast Asian J Trop Med Public Health. 2009 Jan;40(1):30-4.

Abstract

This study described the diagnosis of a mixed infection of Brugia malayi and Brugia pahangi in a single domestic cat using the internal transcribed spacer 1 (ITS1) region. Following polymerase chain reaction amplification of the ITS1 region, the 580 bp amplicon was cloned, and 29 white colonies were randomly selected for DNA sequencing and phylogenetic tree construction. A DNA parsimony tree generated two groups of Brugia spp with one group containing 6 clones corresponding to B. pahangi and the other 23 clones corresponding to B. malayi. This indicated that mixed infection of the two Brugia spp, B. pahangi and B. malayi, had occurred in a single host.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Brugia malayi / classification
  • Brugia malayi / genetics*
  • Brugia malayi / isolation & purification
  • Brugia pahangi / classification
  • Brugia pahangi / genetics*
  • Brugia pahangi / isolation & purification
  • Cat Diseases / diagnosis*
  • Cat Diseases / genetics
  • Cat Diseases / parasitology
  • Cat Diseases / prevention & control
  • Cats
  • DNA, Helminth / analysis
  • DNA, Helminth / genetics*
  • DNA, Ribosomal Spacer / analysis
  • DNA, Ribosomal Spacer / genetics*
  • Disease Reservoirs / veterinary
  • Filariasis / diagnosis
  • Filariasis / parasitology
  • Filariasis / prevention & control
  • Filariasis / veterinary*
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / veterinary
  • Polymorphism, Restriction Fragment Length
  • Sequence Analysis, DNA

Substances

  • DNA, Helminth
  • DNA, Ribosomal Spacer