Abstract
It's raining, it's porin: Fragment ligation of OmpF ion channels was achieved by using the split Psp-GBD Pol intein; this allowed reconstitution of active trimeric porin. In combination with cysteine modification at an internal position, the porin's conductance properties were altered.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cysteine / metabolism
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Electric Conductivity
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Escherichia coli*
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Inteins
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Models, Molecular
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Porins / chemistry*
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Porins / metabolism
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Porosity
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Protein Conformation
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Protein Renaturation
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Protein Splicing*
Substances
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OmpF protein
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Porins
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Cysteine