Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification

Chembiochem. 2009 Apr 17;10(6):983-6. doi: 10.1002/cbic.200900039.

Abstract

It's raining, it's porin: Fragment ligation of OmpF ion channels was achieved by using the split Psp-GBD Pol intein; this allowed reconstitution of active trimeric porin. In combination with cysteine modification at an internal position, the porin's conductance properties were altered.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cysteine / metabolism
  • Electric Conductivity
  • Escherichia coli*
  • Inteins
  • Models, Molecular
  • Porins / chemistry*
  • Porins / metabolism
  • Porosity
  • Protein Conformation
  • Protein Renaturation
  • Protein Splicing*

Substances

  • OmpF protein
  • Porins
  • Cysteine