Protease responsive nanoprobes with tethered fluorogenic peptidyl 3-arylcoumarin substrates

Chem Commun (Camb). 2009 Feb 14:(6):671-3. doi: 10.1039/b816637d. Epub 2008 Dec 8.

Abstract

Protease responsive nanosensors were obtained by the attachment of unique green fluorescent bifunctional 3-arylcoumarin-derived fluorogenic substrates to poly(acrylamide-co-N-(3-aminopropyl)methacrylamide) nanoparticles, in which proteolysis results in substantial signal amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / chemistry
  • Coumarins / chemistry*
  • Cross-Linking Reagents / chemistry
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Kinetics
  • Nanostructures / chemistry*
  • Peptide Hydrolases / metabolism*
  • Peptides / chemistry*
  • Peptides / metabolism*
  • Reproducibility of Results
  • Substrate Specificity

Substances

  • Acrylic Resins
  • Coumarins
  • Cross-Linking Reagents
  • Fluorescent Dyes
  • Peptides
  • polyacrylamide
  • Peptide Hydrolases