Transbronchial needle aspiration biopsy is an endoscopic pulmonary procedure used to diagnose a variety of pulmonary conditions including staging patients with lung cancer and identifying nodules, masses, and benign disorders such as sarcoidosis. Transbronchial needle aspiration biopsy is a safe procedure, performed primarily with local anesthesia combined with intravenous sedation. With the anticipation of this procedure being widely used, more endoscopy nurses will be exposed to this technique. This article is intended to educate nurses and technicians about the procedure of transbronchial needle aspiration and its instruments.