Abstract
We synthesized and determined the production of reactive oxygen species (ROS) as 1O2, *-O2, *OH, H2O2 during the photolysis with UV-A light of three antibacterial quinolones and their naphthyl ester derivatives. Singlet oxygen and ROS dose-dependant generation from norfloxacin (1), enoxacin (2), ciprofloxacin (3) and their respective naphthyl ester derivatives 4-6 were detecting in cell-free systems by the histidine assay and by luminol-enhanced chemiluminescence (LCL). Both the electronic absorption and emission spectra were quantified and their photostability determined. The antibacterial activity in darkness and under irradiation of compounds 4, 5 and 6 was tested on E. coli and compared with their parent drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Anti-Bacterial Agents / radiation effects
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Ciprofloxacin / pharmacology
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Ciprofloxacin / radiation effects
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Culture Media
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Enoxacin / pharmacology
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Enoxacin / radiation effects
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Escherichia coli / drug effects
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Escherichia coli / radiation effects
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Fluoroquinolones / pharmacology*
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Fluoroquinolones / radiation effects
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Histidine / analysis
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Hydrogen Peroxide / chemistry
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Hydrogen-Ion Concentration
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Luminescence
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Naphthalenes / pharmacology*
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Norfloxacin / pharmacology
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Norfloxacin / radiation effects
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Oxidants / chemistry
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Oxidants, Photochemical / pharmacology*
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Photolysis
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Photosensitizing Agents / pharmacology
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Reactive Oxygen Species / chemistry
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Spectrometry, Fluorescence
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Spectrophotometry, Ultraviolet
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Ultraviolet Rays
Substances
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Anti-Bacterial Agents
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Culture Media
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Fluoroquinolones
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Naphthalenes
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Oxidants
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Oxidants, Photochemical
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Photosensitizing Agents
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Reactive Oxygen Species
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Enoxacin
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Histidine
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Ciprofloxacin
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Hydrogen Peroxide
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Norfloxacin