Abstract
Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative As(III) transformation within the microbial mat.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria / genetics*
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Bacteria / isolation & purification
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism*
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Gene Expression Profiling
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Hot Springs / microbiology*
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Microelectrodes
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Molecular Sequence Data
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Oxidoreductases / genetics*
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Oxidoreductases / metabolism*
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Phylogeny
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Analysis, DNA
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Sequence Homology
Substances
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Bacterial Proteins
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DNA, Bacterial
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Oxidoreductases
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arsenite oxidase
Associated data
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GENBANK/FJ376461
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GENBANK/FJ376462
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GENBANK/FJ376463
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GENBANK/FJ376464
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GENBANK/FJ376465
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GENBANK/FJ376466
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GENBANK/FJ376467
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GENBANK/FJ376468
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GENBANK/FJ376469
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GENBANK/FJ376470
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GENBANK/FJ376471
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GENBANK/FJ376472
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GENBANK/FJ376473
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GENBANK/FJ376474
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GENBANK/FJ376475
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GENBANK/FJ376476
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GENBANK/FJ376477
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GENBANK/FJ376478
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GENBANK/FJ376479
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GENBANK/FJ376480
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GENBANK/FJ376481
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GENBANK/FJ376482
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GENBANK/FJ376483