The convergent total synthesis of ganglioside GQ1b based on the "cassette approach" between the nonreducing end GQ1b-core heptasaccharide and glucosylceramide building blocks was accomplished in high overall yield. The use of a sialylalpha(2-->8)sialylalpha(2-->3)galactose sequence as the key building block enhanced the efficiency of the glycan assembly and led to preparative-scale synthesis readily applicable for large-scale preparation. In addition, a judicious choice of p-methoxybenzyl protecting groups on glucosylceramide provided a solution to the previous synthetic problems, including a decrease in the yield of the deprotection steps, and led to elevation of the total yield. Furthermore, unnatural-type GQ1b derivatives were synthesized systematically in good yields by capitalizing on a similar approach in order to elucidate their biological roles.