Detection of selective cationic amphipatic antibacterial peptides by Hidden Markov models

Acta Biochim Pol. 2009;56(1):167-76. Epub 2009 Mar 18.

Abstract

Antibacterial peptides are researched mainly for the potential benefit they have in a variety of socially relevant diseases, used by the host to protect itself from different types of pathogenic bacteria. We used the mathematical-computational method known as Hidden Markov models (HMMs) in targeting a subset of antibacterial peptides named Selective Cationic Amphipatic Antibacterial Peptides (SCAAPs). The main difference in the implementation of HMMs was focused on the detection of SCAAP using principally five physical-chemical properties for each candidate SCAAPs, instead of using the statistical information about the amino acids which form a peptide. By this method a cluster of antibacterial peptides was detected and as a result the following were found: 9 SCAAPs, 6 synthetic antibacterial peptides that belong to a subregion of Cecropin A and Magainin 2, and 19 peptides from the Cecropin A family. A scoring function was developed using HMMs as its core, uniquely employing information accessible from the databases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents / analysis*
  • Anti-Bacterial Agents / chemistry
  • Cations
  • Cluster Analysis
  • Markov Chains*
  • Molecular Sequence Data
  • Peptides / analysis*
  • Peptides / chemistry

Substances

  • Anti-Bacterial Agents
  • Cations
  • Peptides