Application of retrovirus-mediated expression cloning for receptor screening of a parasite

Kyousuke Kobayashi; Kentaro Kato; Tatsuki Sugi; Daisuke Yamane; Masayuki Shimojima; Yukinobu Tohya; Hiroomi Akashi

doi:10.1016/j.ab.2009.03.008

Application of retrovirus-mediated expression cloning for receptor screening of a parasite

Anal Biochem. 2009 Jun 1;389(1):80-2. doi: 10.1016/j.ab.2009.03.008. Epub 2009 Mar 12.

Authors

Kyousuke Kobayashi¹, Kentaro Kato, Tatsuki Sugi, Daisuke Yamane, Masayuki Shimojima, Yukinobu Tohya, Hiroomi Akashi

Affiliation

¹ Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

PMID: 19285477
DOI: 10.1016/j.ab.2009.03.008

Abstract

Retrovirus-mediated expression cloning has been applied in both virology and cell biology. Although there is some difficulty in applying this technique to screening for a receptor recognized by an intracellular parasite, we modified the conventional method to identify a putative receptor for the Plasmodium falciparum BAEBL protein. We show that this method is effective in screening for a parasite receptor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carrier Proteins / metabolism*
Cell Line
Cloning, Molecular / methods*
Flow Cytometry
Glypicans / isolation & purification
Glypicans / metabolism*
Humans
Membrane Proteins
Models, Biological
Plasmodium falciparum / metabolism*
Protein Binding
Protozoan Proteins / metabolism*
Retroviridae / genetics*

Substances

Carrier Proteins
Glypicans
Membrane Proteins
Protozoan Proteins
erythrocyte binding protein-2, Plasmodium falciparum