A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption

Amy L Jenkins; Patricia L Worsham; Susan L Welkos

doi:10.1016/j.mimet.2009.02.013

A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption

J Microbiol Methods. 2009 Jun;77(3):316-9. doi: 10.1016/j.mimet.2009.02.013. Epub 2009 Mar 10.

Authors

Amy L Jenkins¹, Patricia L Worsham, Susan L Welkos

Affiliation

¹ Bacteriology Division, U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA.

PMID: 19281840
DOI: 10.1016/j.mimet.2009.02.013

Abstract

Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm(-) strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes.

Publication types

Evaluation Study
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Bacterial Proteins / genetics
Bacterial Proteins / metabolism
DNA Primers / genetics
Humans
Mutation
Pigments, Biological / genetics*
Pigments, Biological / metabolism
Plague / microbiology*
Polymerase Chain Reaction / methods*
United States
United States Dept. of Health and Human Services / legislation & jurisprudence*
Virulence Factors / genetics
Virulence Factors / metabolism
Yersinia pestis / genetics*
Yersinia pestis / metabolism

Substances

Bacterial Proteins
DNA Primers
Pigments, Biological
Virulence Factors