Objective: To stydy the protective mechanism of Jiedu Tongluo (JDTL) injection on experimental cerebral ischemia.
Method: Radioimmunoassay (RIA) was utilized to identify the content of TNF-alpha and IL1-beta in brain tissue of rats suffered from 6 h and 24 h ischemia with MCAO, Enzyme-linked immunoadsordent assay (ELISA) was used to observe the level of MCP-1 and ICMA-1.
Result: After 6 h and 24 h of ischemia, the increased contents of TNF-alpha, IL1-beta, MCP-1 as well as ICMA-1 was observed. JDTL injection dramatically inhibited the increase of TNF-alpha, IL1-beta after 6 h and 24 h of ischemia.
Conclusion: JDTL injection had inhibiting effect on TNF-alpha, IL1-beta, MCP-1 as well as ICMA-1 caused by cerebral ischemia, the result suggested that JDIL injection can protect cerebral tissue from ischemic injury by inhibiting inflammatory factors.